Diversity of Class 1 Integron Gene Cassette Rearrangements Selected under Antibiotic Pressure
نویسندگان
چکیده
منابع مشابه
Diversity of Class 1 Integron Gene Cassette Rearrangements Selected under Antibiotic Pressure.
UNLABELLED Integrons are bacterial genetic elements able to capture and express genes contained within mobile gene cassettes. Gene cassettes are expressed via a Pc promoter and can be excised from or integrated into the integron by integrase IntI. Although the mechanisms of gene cassette integration and excision are well known, the kinetics and modes of gene cassette shuffling leading to new ge...
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The class 1 integrons are genetic elements capable of integrating gene cassettes by a site-specific recombination mechanism (1). Gene cassettes are mobile units composed of a gene, most often an antibiotic resistance gene, and a recombination site, the 59-base element (1). We have previously characterized a class 1 integron containing a VIM-2-type metallo-lactamase gene, blaVIM-2, an aacA4 gene...
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The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. The integron was located on a plasmid and was identified ...
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The integrase IntI1 catalyses recombination of antibiotic-resistance gene cassettes in the integron, a widely found bacterial mobile element active in spreading antibiotic multi-resistance. We have previously shown that resistance cassette recombination rate and specificity depend on the amount of intracellular integrase. Here, we used in vivo and in vitro methods to examine convergent expressi...
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Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called P2. To pro...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2015
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.02455-14